Background: The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway. RasGRP is a guanyl nucleotide-releasing protein for Ras that contains two EF hand domains, which bind to calcium, and a diacylglycerol (DAG)-binding domain. RasGRP is expressed in the nervous system and lymphoid tissues and may link changes in DAG and calcium concentrations to Ras activation.
Description: Rabbit polyclonal to Rasgrp1
Immunogen: KLH conjugated synthetic peptide derived from Rasgrp1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 90 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.