Background:  Positive transcription elongation factor b (P-TEFb) complexes are crucial for allowing the elongation of RNA by RNA polymerase II (RNAPII). These complexes are able to phosphorylate the carboxyl-terminal domain of the largest RNAPII subunit. P-TEFb complexes are made up of a catalytic subunit, cyclin dependent kinase 9 (Cdk9), and one of the regulatory cyclins, CycT1, CycT2a, CycT2b or cyclin K. Specifically, cyclin K forms an active P-TEFb complex with Cdk9. This complex promotes transcription by phosphorylating the carboxyl-terminal domain of RNAPII which allows the elongation of transcription to proceed. Cyclin K is ubiquitously expressed in adult mouse and human tissues, with highest levels expressed in the developing germ cells of adult testis and ovaries. Cyclin K is also present in HepG2 cells. The cyclin K gene encodes a 357 amino acid protein and maps to human chromosome 14q32.

Description: Rabbit polyclonal to Cyclin Y

Immunogen: KLH conjugated synthetic peptide derived Cyclin Y

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 66 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.