Background: Mitosis is an important process regulated by many proteins. In the event that cellular division is not controlled, cancer, tumors and cellular death become prevalent. HEI-C (enhancer of invasion-cluster) is also known as CCDC5 (coiled-coil domain containing 5 (spindle associated)) and is a 278 amino acid protein expressed as two isoforms. HEI-C is expressed in a variety of tissues including pancreas, kidney, skeletal muscle, liver and heart, where it is localized to the cytoplasm during phases of the cell cycle, excluding mitosis. HEI-C is localized to asters, and is spotted on the microtubule array during metaphase. During the later stages of mitosis, HEI-C remains only on the spindle, then associates with microtubule bundles central to the midbody of the cell. During the metaphase-anaphase transition of mitosis, HEI-C regulates the function and stability of the mitotic spindle. Depletion of HEI-C results in cell death or mitotic delay between metaphase and anaphase, suggesting the importance of functional HEI-C proteins.

Description: Rabbit polyclonal to CCDC5

Immunogen: KLH conjugated synthetic peptide derived from CCDC5

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 32 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.