Background: ARHGAP32 is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase. RICS, also known as GRIT, p200RhoGAP, p250GAP or GC-GAP or ARHGAP32, is a 2,087 amino acid protein that localizes to various regions within the cell, including the cell junction, the endosomal membrane and the endoplasmic reticulum, and contains one SH3 domain, one Rho-GAP domain and one PX domain. Existing as multiple alternatively spliced isoforms that are expressed in a tissue-specific manner, RICS functions as a GTPase-activating protein (GAP) that promotes GTP hydrolysis on a variety of target proteins and is thought to be involved in neuronal differentiation. Additionally, RICS is involved in Actin reorganization in dendritic spines and may participate in Rho- and Ras-regulated signaling pathways in cell growth regulation. Specific RICS isoforms are subject to in vitro phosphorylation, an event which inhibits GAP activity.
Description: Rabbit polyclonal to ARHGAP32
Immunogen: KLH conjugated synthetic peptide derived from ARHGAP32
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 250 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.