Background: The BNPI (brain specific Na(+)-dependent inorganic phosphate(Pi) cotransporter) gene, also designated VGLUT1 (vesicular glutamate transporter), is located on chromosome 19q13 and encodes a 560 amino acid protein with 6-8 transmembrane-spanning domains. BNPI is expressed predominantly in neurons of the cerebral cortex, hippocampus, and cerebellum, and is more highly expressed in adult brain compared to fetal brain. BNPI localizes almost exclusively to nerve terminals forming asymmetric excitatory-type synapses and associates preferentially with the membranes of small synaptic vesicles. In the plasma membrane, BNP1 imports phospate ions, which are required for glutmatergic neurotransmission. Expression of BNPI results in glutamate uptake by intracellular vesicles, which defines a glutamatergic phenotype in neurons.

Description: Rabbit polyclonal to VGLUT1

Immunogen: KLH conjugated synthetic peptide derived from VGLUT1

Specificity:  ·Reacts with Human, Mouse, Dog, Pig, Chicken and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 61 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.