Background: Neurotrophin-1/B cell-stimulating factor-3 (NNT-1/BSF-3, also known as cardiotrophin-like cytokine) is found mainly in lymph nodes and spleen. NNT-1/BSF-3 induces tyrosine phosphorylation of the signal transducing receptor molecule glycoprotein 130 (gp130), leukemia inhibitory factor receptor beta, and signal transducer and activator of transcription 3 in the SK-N-MC human neuroblastoma cells. The activation of gp130 distinguishes a group of cytokines referred to as the IL-6 family. They all show the conserved location of one intron in their gene structure and, in common with cytokines of the hematopoietin superfamily, the presence of a four-helix bundle in their protein structure. In addition to features typical of IL-6 family cytokines, including neurotropic effects, NNT-1/BSF-3 shows B cell-stimulating capability.
Description: Rabbit polyclonal to NNT1
Immunogen: KLH conjugated synthetic peptide derived from NNT1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 25 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.