Background:  DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle; moderate expression in heart, small intestine, liver, pancreas, testis and colon; and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.

Description: Rabbit polyclonal to DPH2

Immunogen: KLH conjugated synthetic peptide derived from DPH2

Specificity:  ·Reacts with Human, Mouse, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 52 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-200;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.