Background:  GATA3 is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.

Description: Rabbit polyclonal to GATA3

Immunogen: KLH conjugated synthetic peptide derived from GATA3

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 50 kDa;

·Immunohistochemistry (Frozen/paraffin tissue section): 1/50-200;

·Immunocytochemistry: 1/100;

·ELISA: 1/500;

· Optimal working dilutions must be determined by the end user.