Background:  ALS2CL is a cytoplasmic protein that acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase, regulating the ALS2-mediated endosome dynamics. It is a homodimer, forming a heteromeric complex with ALS2. It also interacts with RAB5A. ALS2CL is distributed onto the vesicular compartments in the cytoplasm with strong punctated staining. It colocalizes with RAB5A onto the vesicular/membranous compartments in the cytoplasm, particularly to the leading edges of the cells. It is strongly expressed in heart and kidney tissues. 5 isoforms of ALS2CL are produced by alternative splicing. Isoform 1, the canonical form is 108 kDa and 953 amino acids in size.

Description: Rabbit polyclonal to ALS2CL

Immunogen: KLH conjugated synthetic peptide derived from ALS2CL

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 108 kDa;

·Immunohistochemistry (Frozen/paraffin tissue section): 1/50-200;

·Immunocytochemistry: 1/100;

·Optimal working dilutions must be determined by the end user.