Background:  Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. The Mucin 5B gene, which contains a 3’ cis-element, is one of the four mucin genes mapped to human chromosome 11p15. Although Mucin 5B is the prominent human gallbladder mucin, it is also expressed and secreted in the colon. In addition, Mucin 5B is expressed in non-inflammed middle ears and normal esophagus, and is upregulated by chronic inflammation and highly secreted in the diseased middle ear. Mucin 5B is abnormally expressed in gastric carcinomatous tissues. Its expression in gastric cancer cells is controlled by a highly active distal promoter, which is upregulated by protein kinase C and repressed under the influence of methylation. Mucous differentiation and expression of Mucin 5B is retinoic acid- (RA) or retinol-dependent. RA control of mucin gene is mediated by the retinoid acid receptor RARa and, to a lesser extent, by RARg. The correlation of mucin protein levels in human cervical mucous with the peak at midcycle suggests that mucin may be important in sperm transit to the uterus.

Description: Rabbit polyclonal to Muc5B

Immunogen: KLH conjugated synthetic peptide derived from Muc5B

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 590 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.