Background:  PEBP-4 (phosphatidylethanolamine-binding protein 4), also known as Protein cousin-of-RKIP 1 (CORK1), is a 227 amino acid lysosomal protein that is a member of the RAF kinase inhibitory protein (RKIP) family. Unlike other RKIPs, PEBP-4 forms ternary complexes with Raf-1 and MEK and scaffolds this structure, thus resulting in the inhibition of the Raf-1/MEK/ERK signaling pathway. With preferential expression in muscle, PEBP-4 seems to control myocyte differentiation by modulation of MEK and ERK activity. Overexpression of PEBP-4 inhibits TNFα-induced activation of JNK and PE externalization, while reduced expression by siRNA knockdown results in an increase in TNFα-induced apoptosis. This suggests that PEBP-4 promotes cellular resistance to apoptosis and may be implicated in tumorigenesis.

Description: Rabbit polyclonal to PEBP4

Immunogen: KLH conjugated synthetic peptide derived from PEBP4

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 26 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.