Background:  Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. These cytosolic enzymes differ in their tissue distributions and substrate specificities. HNK-1ST, also designated carbohydrate sulfotransferase 10 (CHST10), is a Golgi-associated sulfotransferase that functions in the biosynthesis of HNK-1, a neuronally expressed carbohydrate that harbors a sulfoglucuronyl residue. HNK-1ST and glucuronosyltransferase P (GLCATP) expression is necessary to form the HNK-1 carbohydrate epitope on NCAM, a cell adhesion molecule. HNK-1ST demonstrates prominent expression in adult and fetal brain and adult testis and ovary. The deduced 356 amino acid type II transmembrane protein contains three potential N-glycosylation sites and a conserved RDP sequence that is also present in other Golgi-resident sulfotransferases.

Description: Rabbit polyclonal to CHST10

Immunogen: KLH conjugated synthetic peptide derived from CHST10

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 42 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.