Background: Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the alphaherpesvirus entry mediator. Nectin constitutes a family consisting of at least nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas nectin 1 is abundantly expressed in the brain. Nectin 1 exists as nectin 1å and 1∫/HIgR, produced by alternative splicing. The cytoplasmic regions of Nectin 1å, but not Nectin 1∫/HIgR, have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 1, also designated HveC/ PRR1, allows the entry of herpes simplex virus type 1 (HSV-1) and HSV-2 into mammalian cells. The interaction of virus envelope glycoprotein D (gD) with nectin 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of Nectin 1. Both the transinteraction of nectin and the interaction of nectin with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions.
Description: Rabbit polyclonal to PVRL1
Immunogen: KLH conjugated synthetic peptide derived from PVRL1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 57 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.